YBX1 antibody

Principal name

YBX1 antibody

Alternative names for YBX1 antibody

NSEP1, YB1, CBF-A, EFI-A, Nuclease-sensitive element-binding protein 1, Y-box-binding protein 1, Y-box transcription factor, YB-1, CCAAT-binding transcription factor I subunit A, Enhancer factor I subunit A, DNA-binding protein B

SwissProt ID

P62960 (Mouse), P62961 (Rat), P67808 (Bovin), P67809 (Human), Q06066 (Chick), Q28618 (Rabit)

Gene ID

4904 (YBX1), 22608 (Ybx1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Rb (Rabbit), African clawed frog, Xen (Xenopus), GP (Guinea Pig), Sh (Sheep), Eq (Equine), Por (Porcine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Frozen Sections (C)

Background of YBX1 antibody

YBX1(Y box binding protein 1), commonly referred to as "YB-1" by researchers, is a human protein. YB1 binding has an absolute requirement for the CCAAT box and relative specificity for the Y box. It has a molecular mass of 35,414 and contains 18% basic residues and putative nuclear localization signals. The YBX1 gene is mapped on 1p34.2. Ybx1 was highly expressed in mouse erythroid myeloid lymphoid clone-1 (EML), a hematopoietic precursor cell line, but that it was downregulated in myeloid progenitors and in Gmcsf-treated EML cells during myeloid differentiation. Ybx1 was expressed at high levels in myeloid leukemic cells at different developmental stages. Knockdown of YBX1 in a human leukemic cell line inhibited proliferation ability, induced apoptosis, and induced megakaryocytic differentiation in response to arsenic trioxide treatment. YBX1 is downregulated during myeloid differentiation and aberrant YBX1 expression in leukemic cells may contribute to leukemia development by blocking differentiation.

General readings

Shen Q. et al. (2006) J Cell Physiol. 207:775-783.Chernukhin IV. et al. (2000) J Biol Chem. 275:29915-29921

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

YBX1 antibody

YBX1 antibody - middle region  validated by WB using 1. Human NT-2 cells (60ug) at 2 ug/ml. Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rt, Sh P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

YBX1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-YBX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806164) Mouse IgG1 OTI7B4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

YBX1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-YBX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806283) Mouse IgG1 OTI11A7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

YBX1 antibody

Human Placenta; Placenta; YBX1 antibody - middle region (ARP34396_P050) in Human Placenta cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

YBX1 antibody

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human breast and renal cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using YBX1 antibody at dilution 1/15, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

YBX1 antibody

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human brain and gastric cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using YBX1 antibody at dilution 1/15, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

YBX1 antibody

YBX1 Antibody (Center) (Cat.# TA324685) western blot analysis in 293T cell line lysates (35ug/lane).This demonstrates the YBX1 antibody detected the YBX1 protein (arrow). Rabbit IgG Purified Bov, Hu, Ms, Rb, Rt ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

YBX1 (C-term) antibody

Western blot analysis of YBX1(arrow) using rabbit polyclonal YBX1 Antibody (C-term) (Cat. #TA324830). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the YBX1 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

YBX1 antibody

WB Suggested Anti-NSEP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysateYBX1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

YBX1 antibody

Western Blot: YB1 Antibody [TA336872] - Western blot analysis of YB1 expression in 1) HeLa and 2) NTera2 whole cell lysates using TA336872. Rabbit Purified Hu, Ms, Rt C, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

YBX1 antibody

Host: Rabbit; Target Name: YBX1; Sample Tissue: Thymus Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

YBX1 antibody

Western Blot: YB1 Antibody  Western Blot was performed using affinity purified SEQer YB-1 antibody, aa(1-55) antibody. The lanes contain 5-30ug of a whole cell extract.  Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer YB-1 antibody, aa(1-55) antibody.  Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec).  The protein was detected as represented by the band shown. Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

YBX1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY YBX1 (RC209835, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-YBX1. Mouse IgG1 OTI2C9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

YBX1 (N-term) antibody

(LEFT)Western blot analysis of anti-YBX1 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane).YBX1(arrow) was detected using the purified Pab (1:60dilution). (RIGHT)Western blot analysis of YBX1 (arrow) using rabbit polyclonal YBX1 Antibody (N-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the YBX1 gene (Lane 2) Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

YBX1 antibody

YBX1 Rabbit Serum Ms C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

YBX1 pSer102 antibody

Western blot analysis of extracts from HepG2 cells treated with PMA using YB1 (Phospho-Ser102) Antibody AP55836PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

YBX1 pSer102 antibody

Western blot analysis of extracts from HepG2 cells treated with PMA using YB1 (Phospho-Ser102) Antibody AP55836PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €270.00
  Acris Antibodies GmbH

YBX1 antibody

YBX1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

YBX1 antibody

Western Blot analysis of YBX1 expression in transfected 293T cell line (H00004904-T01) by YBX1 MaxPab polyclonal antibody.

Lane 1: YBX1 transfected lysate(35.90 KDa).
Lane 2: Non-transfected lysate. Rabbit Serum Hu E(detection), WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

YBX1 antibody

Immunofluorescence of monoclonal antibody to YBX1 on HeLa cell. [antibody concentration 20 ug/ml] Mouse IgG2a 4F12 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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