YY1 antibody

Principal name

YY1 antibody

Alternative names for YY1 antibody

Yin and yang 1, YY-1, INO80S, NF-E1, Transcriptional repressor protein YY1, Delta transcription factor, INO80 complex subunit S

SwissProt ID

P25490 (Human), Q00899 (Mouse)

Gene ID

7528 (YY1), 22632 (Yy1)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), Por (Porcine), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of YY1 antibody

YY1 (UniProtKB/Swiss-Prot entry P25490) is a multifunctiol transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. The consensus sequence is 5'-CCGCCATNTT-3', although some genes have been shown to contain a longer binding motif allowing enhanced binding. The initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which YY1 binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformatiol D changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. YY1 may play an important role in development and differentiation.

General readings

Cai Y., et al (2007) Nat. Struct. Mol. Biol. 14:872-874 Wu S., et al (2007) Nat. Struct. Mol. Biol. 14:1165-1172

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

YY1 antibody

Human, Testis: Formalin-Fixed Paraffin-Embedded (FFPE) Mouse IgG1 4D2 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

YY1 antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 4A5 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

YY1 (221-321) antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 2C4 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

YY1 (N-term) antibody

  Rabbit polyclonal anti-YY1 antibody(N-term), Loading control  
YY1 Antibody (L89) (Cat. #TA324719) western blot analysis in 293,HL-60,SiHa,K562,THP-1,U266 cell line lysates (35ug/lane).This demonstrates the YY1 antibody detected the YY1 protein (arrow). Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

YY1 antibody

  Rabbit Polyclonal Anti-YY1 Antibody  
WB Suggested Anti-YY1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Thymus Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

YY1 antibody

  Rabbit Polyclonal Anti-YY1 Antibody  
WB Suggested Anti-YY1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Thymus Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

YY1 antibody

  Rabbit Polyclonal Anti-YY1 Antibody  
WB Suggested Anti-YY1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate; There is BioGPS gene expression data showing that YY1 is expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

YY1 antibody

  Rabbit Polyclonal YY1 Antibody  
Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against YY1. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:4,800. Rabbit IgG Purified Hu, Ms E
0.1 ml / €325.00
  OriGene Technologies, Inc.

YY1 (Center) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Yy1 - mouse gene knockout kit via CRISPR

Yy1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Yy1 - mouse gene knockout kit via CRISPR

Yy1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Yy1 - mouse gene knockout kit via CRISPR

Yy1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Yy1 - mouse gene knockout kit via CRISPR

Yy1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

YY1 (1-414, His-tag)

YY1 Human Purified > 80 % by SDS - PAGE E. coli
0.5 mg / €940.00
  Acris Antibodies GmbH

YY1 (1-414, His-tag)

YY1 Human Purified > 80 % by SDS - PAGE E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

YY1 overexpression lysate

YY1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, YY1 (mGFP-tagged) - Human YY1 transcription factor (YY1), 200 uL, >10^7 TU/mL

Lenti ORF particles, YY1 (mGFP-tagged) - Human YY1 transcription factor (YY1), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, YY1 (Myc-DDK tagged) - Human YY1 transcription factor (YY1) , 200 uL, >10^7 TU/mL

Lenti ORF particles, YY1 (Myc-DDK tagged) - Human YY1 transcription factor (YY1) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

18 Item(s)

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