ZA20D1 antibody
Principal name
ZA20D1 antibody
Alternative names for ZA20D1 antibody
OTUD7B, Cezanne, OTU domain-containing protein 7B, Zinc finger A20 domain-containing protein 1, Cellular zinc finger anti-NF-kappa-B protein
SwissProt ID
Q6GQQ9 (Human)
Gene ID
56957 (OTUD7B)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ZA20D1 antibody
OTUD7B has deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. Hydrolyzes both linear and branched forms of polyubiquitin. Negative regulator of nuclear factor NF-kappa-B.
OTUD7B is Widely expressed. Abundant in kidney, heart and fetal liver. Expressed differentially among B-cells at distinct developmental stages. Higher expression seen in primary immature B-cells as compared to the mature cells.
Sequence similarities:
Belongs to the peptidase C64 family.
Contains 1 A20-type zinc finger.
Contains 1 OTU domain.
General readings
Evans,P.C., et al., (2003) J Biol Chem. 278(25), 23180-6
Human Daudi; WB Suggested Anti-ZA20D1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: Daudi cell lysate. OTUD7B is supported by BioGPS gene expression data to be expressed in Daudi.; ZA20D1 antibody - middle region (ARP34593_T100) in Human Daudi cells using Western Blot
Immunoperoxidase of monoclonal antibody to OTUD7B on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml]
Western Blot analysis of OTUD7B expression in transfected 293T cell line (H00056957-T01) by OTUD7B MaxPab polyclonal antibody.Lane 1: ZA20D1 transfected lysate(46.97 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of OTUD7B expression in transfected 293T cell line (H00056957-T01) by OTUD7B MaxPab polyclonal antibody.Lane 1: ZA20D1 transfected lysate(46.97 KDa).Lane 2: Non-transfected lysate.
Western Blot: ZA20D1 Lysate [NBL1-14013] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for OTUD7B
Immunohistochemical of paraffin-embedded human kidney using 16605-1-AP(OTUD7B antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human kidney using 16605-1-AP(OTUD7B antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using 16605-1-AP(OTUD7B antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using 16605-1-AP(OTUD7B antibody) at dilution of 1:25 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using 16605-1-AP(OTUD7B antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human kidney using 16605-1-AP(OTUD7B antibody) at dilution of 1:25 (under 40x lens)
Immunohistochemical of paraffin-embedded human heart using 16605-1-AP(OTUD7B antibody) at dilution of 1:25 (under 40x lens)
Immunohistochemical of paraffin-embedded human kidney using 16605-1-AP(OTUD7B antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using 16605-1-AP(OTUD7B antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded human heart using 16605-1-AP(OTUD7B antibody) at dilution of 1:25 (under 10x lens)
Immunohistochemical of paraffin-embedded mouse kidney using 16605-1-AP(OTUD7B antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded mouse heart using 16605-1-AP(OTUD7B antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded mouse heart using 16605-1-AP(OTUD7B antibody) at dilution of 1:25 (under 10x lens)
Immunohistochemical of paraffin-embedded mouse heart using 16605-1-AP(OTUD7B antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded mouse heart using 16605-1-AP(OTUD7B antibody) at dilution of 1:25 (under 40x lens)
Immunohistochemical of paraffin-embedded mouse kidney using 16605-1-AP(OTUD7B antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemical of paraffin-embedded mouse kidney using 16605-1-AP(OTUD7B antibody) at dilution of 1:25 (under 10x lens)
Immunohistochemical of paraffin-embedded mouse kidney using 16605-1-AP(OTUD7B antibody) at dilution of 1:25 (under 40x lens)
Jurkat cells were subjected to SDS PAGE followed by western blot with 16605-1-AP(OTUD7B antibody) at dilution of 1:600
Jurkat cells were subjected to SDS PAGE followed by western blot with 16605-1-AP(OTUD7B antibody) at dilution of 1:800
IP Result of anti-OTUD7B (IP:16605-1-AP, 4ug; Detection:16605-1-AP 1:800) with Jurkat cells lysate 2500ug.
Human 293T; WB Suggested Anti-OTUD7B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate; OTUD7B antibody - middle region (ARP34594_P050) in Human 293T cells using Western Blot
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Detection limit for recombinant GST tagged OTUD7B is 0.3 ng/ml as a capture antibody.
Immunohistochemistry-Paraffin: ZA20D1 Antibody [NBP1-88095] - Immunohistochemical staining of human rectum shows strong cytoplasmic and nuclear positivity in glandular cells.
Immunofluorescence: ZA20D1 Antibody [NBP1-88095] - 251MG shows positivity in cytoskeleton (microtubules).
Western Blot: ZA20D1 Antibody [NBP1-88095] Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA337240 | ZA20D1 antibody |
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 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt | WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA337241 | ZA20D1 antibody |
||||||
 |
Rabbit | IgG | Purified | Bov, Can, GP, Hu, Ms, Por, Rt, Ze | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| AM31751PU-N | ZA20D1 antibody |
||||||
 |
Mouse | IgG2b | 2B4 | Purified | Hu | E, P, WB |
50 µg /
€530.00
|
| Acris Antibodies GmbH | |||||||
 +1 additional image |
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| H00056957-B01 | ZA20D1 antibody |
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 |
Mouse | Serum | Hu | E(detection), WB |
50 µl /
€370.00
|
||
| Abnova Taiwan Corp. | |||||||
| H00056957-B01P | ZA20D1 antibody |
||||||
 |
Mouse | Purified | Hu | E(detection), WB |
50 µg /
€370.00
|
||
| Abnova Taiwan Corp. | |||||||
| H00056957-M01 | ZA20D1 antibody |
||||||
![Immunoperoxidase of monoclonal antibody to OTUD7B on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml]](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/H00056957-M01-3-46-1-L.jpg) |
Mouse | IgG2b | 2B4 | Purified | Hu | E, P, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| ARP34593_T100 | ZA20D1 antibody |
||||||
 |
Rabbit | Aff - Purified | Bov, Can, Hu, Ms, Rt | WB |
0.1 mg /
€310.00
|
||
| AVIVA Systems Biology | |||||||
| ARP34594_P050 | ZA20D1 antibody |
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 |
Rabbit | Aff - Purified | Bov, Can, Hu, Ms, Rt | WB |
50 µg /
€350.00
|
||
| AVIVA Systems Biology | |||||||
| NBP1-88095 | ZA20D1 antibody |
||||||
![Immunohistochemistry-Paraffin: ZA20D1 Antibody [NBP1-88095] - Immunohistochemical staining of human rectum shows strong cytoplasmic and nuclear positivity in glandular cells.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/ZA20D1 Antibody-Immunohistochemistry-Paraffin-NBP1-88095-0005.jpg) |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€500.00
|
|
| Novus Biologicals Inc. | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309932 | ZA20D1 |
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 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
| H00056957-P01-10 | ZA20D1 |
||||
 |
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
10 µg /
€380.00
|
|
| Abnova Taiwan Corp. | |||||
| H00056957-P01-25 | ZA20D1 |
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|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
25 µg /
€510.00
|
|
| Abnova Taiwan Corp. | |||||
| H00056957-Q01-10 | ZA20D1 |
||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
10 µg /
€380.00
|
|
| Abnova Taiwan Corp. | |||||
| H00056957-Q01-25 | ZA20D1 |
||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
25 µg /
€510.00
|
|
| Abnova Taiwan Corp. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| H00056957-T01 | OTUD7B 293T Cell Transient Overexpression Lysate(Denatured) |
|
|
0.1 ml /
€270.00
|
|
| Abnova Taiwan Corp. |
Lysates (Overexpressed)
| Catalog No. | ||
|---|---|---|
| LY412591 | OTUD7B overexpression lysate |
|
|
0.1 mg /
€280.00
|
|
| OriGene Technologies, Inc. |
