ZAP70 antibody

Principal name

ZAP70 antibody

Alternative names for ZAP70 antibody

ZAP-70, ZAP 70, SRK, 70 kDa zeta-associated protein, Syk-related tyrosine kinase

SwissProt ID

P43403 (Human), P43404 (Mouse)

Gene ID

7535 (ZAP70), 5395 (PMS2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Mky (Monkey), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of ZAP70 antibody

The ZAP70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP70 gene mutations resulting in lower ZAP70 protein expression levels or expression of catalytically inactive ZAP70 proteins, have been identified. ZAP70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency. In patients with chronic lymphocytic leukemia (B-CLL), ZAP70 expression on B cell was shown to be correlated with disease progression and survival.
ZAP70 is cytosolic protein migrating at 70 kDa in SDS-PAGE. It contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. Crystal structure of the ZAP70 SH2 domains in complex with a TCR zeta subunit peptide was described. During T cell activation , the binding of ZAP70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP70 on Tyr493 in the activation loop. ZAP70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.

General readings

General / background references:van Oers NS et al. Production and characterization of monoclonal antibodies specific for the murine T cell receptor zeta chain. J Immunol Methods 170:261-8 (1994). PubMed PMID: 7512608

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ZAP70 (C-term) antibody

Human tonsil stained with Anti-ZAP-70, Cat.  AP15862PU Rabbit Purified Hu P, WB
0.5 ml / €410.00
  Acris Antibodies GmbH

ZAP70 (C-term) antibody

Human tonsil stained with Anti-ZAP-70, Cat.  AP15862PU Rabbit Purified Hu P, WB
1 ml / €520.00
  Acris Antibodies GmbH

ZAP70 (C-term) antibody

Human tonsil stained with Anti-ZAP-70, Cat.  AP15862PU Rabbit Purified Hu P, WB
0.1 ml / €190.00
  Acris Antibodies GmbH

ZAP70 antibody


AP31926PU-N ZAP70 antibody staining of Jurkat lysate at 0.3 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

ZAP70 antibody

Western blot analysis of extracts from Jurkat cells, using ZAP-70 (Ab-319) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ZAP70 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ZAP-70 (Ab-493) Antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ZAP70 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ZAP-70 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ZAP70 antibody

Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using ZAP-70 (Ab-292) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ZAP70 antibody

Predicted band size: 69 kDa . Positive control: HL60 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ZAP70 antibody

ZAP70 Antibody (Center) (Cat. #TA324539) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ZAP70 antibody detected the ZAP70 protein (arrow). Rabbit IgG Purified Hu F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

ZAP70 antibody

Western blot analysis of ZAP-70 expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ZAP70 antibody

Immunohistochemistry staining of Paraffin Chronic lymphocytic leukemia tissue by Zap-70 antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic) Mouse IgG2a 2F3.2 Purified Hu P
1 ml / €160.00
  OriGene Technologies, Inc.

ZAP70 antibody

ZAP70 Rabbit IgG Purified Hu, Ms, Rt P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ZAP70 antibody

Western blot analysis of extracts of various cell lines, using ZAP70 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

ZAP70 antibody

Western blot detection of ZAP-70 in CEM and Jurkat cell lysates,negative in the Ramos cell lysates using ZAP-70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa. Mouse IgG2b 4B2-C1-H3 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ZAP70 antibody

ZAP70 Mouse IgG1 SBZAP FITC Hu E
0.1 mg / €400.00
  Acris Antibodies GmbH

ZAP70 antibody

ZAP70 Mouse IgG1 SBZAP FITC Hu
25 µg / €230.00
  Acris Antibodies GmbH

ZAP70 antibody

ZAP70 Mouse IgG1 SBZAP Purified Hu E
25 µg / €240.00
  Acris Antibodies GmbH

ZAP70 antibody

ZAP70 Mouse IgG1 SBZAP PE Hu F
0.1 mg / €410.00
  Acris Antibodies GmbH

ZAP70 antibody

Figure 2. Western Blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell leukemia cell line (1, 3) and RAMOS human Burkitt lymphoma cell line (2, 4); Lane 1, 2: immunostaining with anti-ZAP70 (ZAP-03; 0.5 ?g/ml). Lane 3, 4: immunostaining with Isotype mouse IgG1 control (PPV-06; AM03095PU-N) Mouse IgG1 ZAP-03 Aff - Purified Hu F, ICC/IF, WB
0.1 mg / €230.00
  Acris Antibodies GmbH

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