ZBTB7A / Pokemon antibody

Principal name

ZBTB7A / Pokemon antibody

Alternative names for ZBTB7A / Pokemon antibody

ZBTB7, FBI1, FBI-1, LRF, TIP21, Zinc finger and BTB domain-containing protein 7A, POK (POZ and Krueppel) erythroid myeloid ontogenic factor, Factor binding IST protein 1, Leukemia/lymphoma-related factor, TTF-I-interacting peptide 21

SwissProt ID

O88939 (Mouse), O93567 (Chick), O95365 (Human), Q9QZ48 (Rat)

Gene ID

51341 (ZBTB7A), 16969 (Zbtb7a)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Chk (Chicken), African clawed frog, GP (Guinea Pig), Ze (Zebrafish), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of ZBTB7A / Pokemon antibody

Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.

General readings

Laudes,M., et al., (2004) J. Biol. Chem. 279 (12), 11711-11718

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ZBTB7A / Pokemon antibody

WB Suggested Anti-ZBTB7A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ZBTB7A / Pokemon antibody

Nuclear and cytoplasmic staining of reactive tonsil (Human) is shown on Formalin Fixed Paraffin tissue. The sections were stained at 5 µg/ml with Pokemon Antibody Cat.-No AM32711PU-N (Clone BC17-2.9F) and peroxidase conjugated anti-Mouse Ig secondary antibody with DAB substrate. Mouse IgG1 BC17-2.9F Purified Hu C, ICC/IF, P, WB
0.1 mg / €250.00
  Acris Antibodies GmbH

ZBTB7A / Pokemon antibody

Hum. Adult Placenta; Host:Rabbit. Target Name:ZBTB7A. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; ZBTB7A antibody - N-terminal region (ARP37762_P050) in Hum. Adult Placenta cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

ZBTB7A / Pokemon (N-term) antibody

Western blot analysis of ZBTB7A in human ovary tissue lysate with ZBTB7A antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ZBTB7A / Pokemon antibody

WB Suggested Anti-ZBTB7A Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:12500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ZBTB7A / Pokemon antibody

Host: Rabbit; Target Name: ZBTB7A; Sample Tissue: Liver Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ZBTB7A / Pokemon antibody

WB Suggested Anti-ZBTB7A Antibody Titration: 5.0 ug/ml; ELISA Titer: 1:1562500; Positive Control: NIH/3T3 cell lysate Rabbit IgG Purified Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ZBTB7A / Pokemon antibody

WB Suggested Anti-ZBTB7A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ZBTB7A / Pokemon (C-term) antibody

Formalin-Fixed Paraffin-Embedded Human Spleen stained with ZBTB7A / Pokemon antibody Cat.-No AP08834PU-N at 5 µg/ml after heat-induced antigen retrieval. Goat IgG Aff - Purified Hu, Mky, Prim E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ZBTB7A / Pokemon Control Peptide

ZBTB7A / Pokemon
50 µg / €160.00
  Acris Antibodies GmbH

ZBTB7A / Pokemon antibody

Immunofluorescence of purified MaxPab antibody to ZBTB7A on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

ZBTB7A / Pokemon antibody

ZBTB7A / Pokemon Mouse IgG2a 2A2 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

ZBTB7A / Pokemon antibody

ZBTB7A / Pokemon Rabbit Hu, Ms IP, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

ZBTB7A / Pokemon antibody

Human HepG2; WB Suggested Anti-ZBTB7A Antibody Titration: 1.25ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate; ZBTB7A antibody - middle region (ARP35899_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

ZBTB7A / Pokemon antibody

Human HeLa; WB Suggested Anti-ZBTB7A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Hela cell lysate; ZBTB7A antibody - N-terminal region (ARP39211_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

ZBTB7A / Pokemon antibody

Western Blot and IP: Pokemon Antibody [NB100-761] - Detection of Human and Mouse ZBTB7(FBI-1) on HeLa whole cell lysate using NB100-761. ZBTB7(FBI-1) was also immunoprecipitated using rabbit antibodies Another ZBTB7(FBI-1) Ab and NB100-762. Rabbit Aff - Purified Hu, Ms IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

ZBTB7A / Pokemon antibody

Western Blot and IP: Pokemon Antibody [NB100-762] - Detection of Human ZBTB7(FBI-1) on HeLa whole cell lysate using NB100-762. ZBTB7(FBI-1) was also immunoprecipitated using rabbit antibodies NB100-761 and another ZBTB7(FBI-1) Ab. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ZBTB7A / Pokemon

ZBTB7A / Pokemon Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ZBTB7A / Pokemon

ZBTB7A / Pokemon Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ZBTB7A / Pokemon

ZBTB7A / Pokemon Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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