ZC3H8 antibody
Principal name
ZC3H8 antibody
Alternative names for ZC3H8 antibody
ZC3HDC8, Fliz1, Zinc finger CCCH domain-containing protein 8
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ZC3H8 antibody
Acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene (By similarity). Induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.
General readings
1, Lamesch, P., et al. Genomics 89(3):307-315(2007).
2. Hwang, E.S., et al. Immunology 106(4):464-469(2002).
3. Hwang, E.S., et al. J. Immunol. 169(1):248-253(2002).
ZC3H8 Antibody (N-term) (AP54623PU-N) western blot analysis in mouse Neuro-2a cell line lysates (35 µg/lane). This demonstrates the ZC3H8 antibody detected the ZC3H8 protein (arrow).
Human Lung, Smooth Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of extracts from HepG2 cells, using ZC3H8 Antibody Cat.-No AP32303PU-N. The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of ZC3H8 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of ZC3H8 expression in HepG2 (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZC3H8 (RC204522, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZC3H8.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZC3H8 (RC204522, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZC3H8.(1:2000)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ZC3H8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808289)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ZC3H8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808289)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ZC3H8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808289)(1:150)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ZC3H8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808289)(1:150)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ZC3H8 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808289)(1:150)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ZC3H8 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808289)(1:150)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ZC3H8 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808289)(1:150)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-ZC3H8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808289)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ZC3H8 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808289)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ZC3H8 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808290)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ZC3H8 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808290)(1:150)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ZC3H8 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808290)(1:150)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-ZC3H8 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808290)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZC3H8 (RC204522, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZC3H8.(1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZC3H8 (RC204522, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZC3H8.(1:2000)
Sample(30 ug whole cell lysate). A:A431. B:Hep G2 . C:Raji . 12% SDS PAGE. TA309043 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using ZC3H8(TA309043) antibody at 1:500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZC3H8(TA309043) antibody at 1:200 dilution.
Western blot analysis of extracts from HepG2 cells, using ZC3H8 antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using ZC3H8 antibody.The picture on the right is treated with the synthesized peptide.
