ZEB1 antibody

Principal name

ZEB1 antibody

Alternative names for ZEB1 antibody

AREB6, TCF8, TCF-8, NIL-2-A, Zinc finger E-box-binding homeobox 1, Negative regulator of IL2, Transcription factor 8, NIL-2-A zinc finger protein

SwissProt ID

P36197 (Chick), P37275 (Human), Q60542 (Mesau), Q62947 (Rat), Q64318 (Mouse)

Gene ID

6935 (ZEB1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), African clawed frog, Prim (Primate)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Electrophoretic Mobility Shift Assay (EMSA), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ChIP assay (ChIP)

Background of ZEB1 antibody

ZEB1, also known as AREB6, is a zinc finger transcription factor encoding protein that represses T-lymphocyte-specific interleukin-2 (IL-2) gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (PMID: 1840704). ZEB1 is expressed in both skeletal and heart muscle, but is not found in the liver. ZEB1 mutations can cause posterior polymorphous corneal dystrophy type 3 (PPCD3) as well as corneal dystrophy Fuchs endothelial type 6 (FECD6). The former is a condition characterized by the metaplasia and overgrowth of corneal endothelial cells thus leading to an increased risk for glaucoma. The latter ocular disorder, FECD6, leads to reduced vision and pain and is caused by loss of endothelium of the central cornea. ZEB1 antibodies are useful tools for studying certain degenerative ocular diseases.

General readings

Park SM, Gaur AB, Lengyel E, Peter ME.
The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2.
Genes Dev. 2008 Apr 22 (7): 894-907.
PMID: 18381893

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ZEB1 antibody

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802298) Mouse IgG2a OTIG6 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ZEB1 antibody

Western blot analysis of extracts of various cell lines, using ZEB1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

ZEB1 antibody

Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802313) Mouse IgG2a OTI7E12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ZEB1 antibody

Human Eye; . Sample Type : Eye tissue .; ZEB1 antibody - N-terminal region (ARP32422_P050) in Human Eye cells using Immunofluorescence Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

ZEB1 (both Isoforms) antibody

TA305688 (0.05ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ZEB1 antibody

Western blot analysis of HEK293 cell lysates with anti-AREB6 antibody (1:100). Rabbit Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ZEB1 (Center) antibody

Western blot analysis of ZEB1 in HeLa cell lysate with ZEB1 antibody at 1ug/mL. Rabbit Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ZEB1 antibody

Western blot analysis of MCF7 cell and PC-3 cell lysate using ZEB1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

ZEB1 antibody

Western Blot: ZEB1 Antibody [TA336897] - WB analysis of Zeb1 in 1) HeLa  2) Cos7 3) HepG2 and 4) Ntera2 cell lysates. Rabbit Purified Hu, Prim ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ZEB1 antibody

Immunohistochemistry: ZEB1 Antibody (2A8A6) [TA336943] - IHC analysis of paraffin-embedded human cervical cancer tissues with DAB staining. Mouse IgG1 2A8A6 Purified Hu C, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ZEB1 antibody

Western Blot: TCF8 Antibody - Western blot detection of TCF8 in U2OS cell lysate. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ZEB1 antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA802295) Mouse IgG2a OTI8E2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ZEB1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (RC217704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1. Mouse IgG2b OTI8A2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ZEB1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ZEB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA802309) Mouse IgG1 OTI9A3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ZEB1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (RC217704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1. Mouse IgG2a OTI5B8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ZEB1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (RC217704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1. Mouse IgG2b OTI10A12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ZEB1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (RC217704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1. Mouse IgG1 OTI8H8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ZEB1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (RC217704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1. Mouse IgG1 OTI4B8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ZEB1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB1 (RC217704, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB1. Mouse IgG2a OTI9G3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ZEB1 antibody

Formalin-Fixed, Paraffin-Embedded Human Heart stained with ZEB1 Antibody Cat.-No AP55676PU-N Rabbit IgG Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

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