ZFHX1B antibody

Principal name

ZFHX1B antibody

Alternative names for ZFHX1B antibody

ZFX1B, ZEB2, SMADIP1, SIP1, KIAA0569, Zinc finger homeobox protein 1b, Zinc finger E-box-binding homeobox 2, Smad-interacting protein 1

SwissProt ID

O60315 (Human), Q9R0G7 (Mouse)

Gene ID

9839 (ZEB2)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Can (Canine), Mky (Monkey), Rb (Rabbit), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C)

Background of ZFHX1B antibody

ZEB2 (Zinc finger E-box-binding homeobox2), also known as SIP1or ZINC FINGER HOMEOBOX 1B (ZFHX1B), is a protein that in humans is encoded by the ZEB2 gene. The ZEB2 gene is a member of the ZEB1/Drosophila Zfh1 family of 2-handed zinc finger/homeodomain proteins and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs, the transducers of TGF-beta signaling, and interacts with the nucleosome remodeling and histone deacetylation (NURD) complex. By radiation hybrid analysis, Nagase et al. (1998) mapped the ZEB2 gene to chromosome 2. Wakamatsu et al. (2001) mapped the ZEB2 gene to chromosome 2q22. Vandewalle et al. (2005) showed that expression of mouse Sip1 in human epithelial cells caused a morphologic change from an epithelial to a mesenchymal phenotype. Expression of SNAI1 in epithelial cells triggers an epithelial-mesenchyme transition. Beltran et al. (2008) showed that synthesis of ZEB2 was upregulated following SNAI1 expression in human cell lines.

General readings

Verstappen,G., (2008) Hum. Mol. Genet. 17 (8), 1175-1183

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ZFHX1B antibody

Immunohistochemistry analysis of SIP1 antibody Cat.-No AP01369PU-N in paraffin-embedded human brain tissue Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

ZFHX1B antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ZEB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802113) Mouse IgG1 OTIE12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ZFHX1B (545-558) antibody

(0.2ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ZFHX1B antibody

Western blot analysis of extracts from HepG2 cells, using ZEB2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ZFHX1B (C-term) antibody

Western blot analysis of ZEB2 in Jurkat cell lysate with ZEB2 antibody at (A) 1 and (B) 2ug/mL. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ZFHX1B antibody

WB Suggested Anti-ZFHX1B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human brain Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ZFHX1B antibody

WB Suggested Anti-ZEB2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Por, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ZFHX1B antibody

WB Suggested Anti-ZEB2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Eq, GP, Hu, Ms, Por, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ZFHX1B antibody

Western blot analysis of ZEB2 expression in HepG2 cell lysate Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ZFHX1B antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ZEB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802108) Mouse IgG1 OTI15G8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ZFHX1B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB2 (RC215227, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB2. Mouse IgG1 OTID12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ZFHX1B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB2 (RC215227, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB2. Mouse IgG1 OTI3D8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ZFHX1B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZEB2 (RC215227, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB2. Mouse IgG1 OTI4G3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ZFHX1B (C-term) antibody

Human Small Intestine, Submucosal Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ZFHX1B (N-term) antibody

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ZFHX1B (C-term) antibody

ZEB2 Antibody (C-term) (AP54642PU-N) western blot analysis in MDA-MB435 cell line lysates (35 µg/lane). This demonstrates the ZEB2 antibody detected the ZEB2 protein (arrow). Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ZFHX1B antibody

Western Blot analysis of ZEB2 expression in transfected 293T cell line (H00009839-T02) by ZEB2 MaxPab polyclonal antibody.

Lane 1: ZEB2 transfected lysate(136.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ZFHX1B antibody

Western Blot analysis of ZFHX1B expression in transfected 293T cell line by ZFHX1B monoclonal antibody (M01), clone 2D9.

Lane 1: ZFHX1B transfected lysate(136.447 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 2D9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ZFHX1B antibody

ZFHX1B monoclonal antibody (M02), clone 2H5. Western Blot analysis of ZFHX1B expression in human ovary cancer. Mouse IgG2b 2H5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ZFHX1B antibody

Immunofluorescence of monoclonal antibody to ZFHX1B on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 2D12 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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