Bov (Bovine), Can (Canine), Hu (Human), Por (Porcine), Chk (Chicken), Ms (Mouse), Rt (Rat), GP (Guinea Pig), Eq (Equine)
Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)
Background of ZFP64 (isoforms 1 and 2) antibody
ZFP64 belongs to the krueppel C2H2-type zinc-finger protein family. It contains 9 C2H2-type zinc fingers. ZFP64 may be involved in transcriptional regulation. Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human ZFP64 with an internal ID of P04189
Suzuki,Y., Gene 200 (1-2), 149-156 (1997)
Transfected 293T; WB Suggested Anti-ZFP64 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; ZFP64 antibody - N-terminal region (ARP39337_T100) in Transfected 293T cells using Western Blot
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using ZFP64(GTX107214) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Western Blot: ZFP64 Antibody [NBP1-78187]-Whole cell lysate from 293T (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg), HeLa (H; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-ZFP64 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ZFP64 was also immunoprecipitated by another rabbit anti-ZFP64 antibody which recognizes a downstream epitope.