ZMAT3 antibody

Principal name

ZMAT3 antibody

Alternative names for ZMAT3 antibody

WIG1, PAG608, Zinc finger matrin-type protein 3, Zinc finger protein WIG-1, p53-activated gene 608 protein

SwissProt ID

O08781 (Rat), O54836 (Mouse), Q0IIC4 (Bovin), Q9HA38 (Human)

Gene ID

64393 (ZMAT3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of ZMAT3 antibody

Human cancer is a multistep process in which accumulation of genetic damage occurs to key regulatory genes, known as oncogenes, and to tumorsuppressor genes. The wild-type (wt) p53 gene is classified as a tumor-suppressor gene (Levine et al). Point mutations in the wild-type gene has been suggested as a key event in the development of malignancy, since the mutant protein actually acts as a dominant oncogene. Indeed, mutations of the p53 gene are the most common molecular changes identified in human cancer; they have been reported to be a frequent feature of breast, lung, colon, ovarian, brain, testicular and bladder cancers, melanoma, neurofibrosarcoma and certain types of leukemia (Midgley et al). In all these cases, mutations are found only in the tumor tissue and not in the normal tissue. A major outcome of such mutations is inactivation of the biochemical and biological functions of the wt p53 protein (Bates et al). Among the biological effects elicited by wt p53, the most documented are cell cycle arrest, apoptosis and its ability to act as a transcriptional regulator. p53 is a transcription factor capable of binding in a sequence-specific manner to well defined DNA elements and inducing the transcription of genes residing in the vicinity of such p53 response elements (Israeli et al). Also, p53 can repress the transcription of many genes, although direct sequence specific interactions between p53 and these genes are generally not involved in this process. Sequence specific transactivation (SST) can either be obligatory or dispensable for apoptosis. Though, it is most likely that an optimal apoptotic effect of p53 requires a combination of both SST and other SST-independent biochemical activities. Among the target genes whose sequence-specific transactivation by wt p53 may promote apoptosis are: bax, the gene coding IGF-BP3, the mammalian homologue of the Drosophila seven in absentia gene, and PAG608. PAG608, a novel p53 target gene, encodes a nuclear zinc finger protein whose overexpression promotes apoptosis in transfected human tumor-derived cells. PAG608 exhibits a predominantly nuclear localization. The staining pattern of the transfected protein suggests that it is localized preferentially to nucleoli. This is compatible with a possible role in regulation of rRNA synthesis by mediating a particular inhibitory effects through interference with optimal production of new ribosomes. The conspicuous induction of PAG608 in cells undergoing p53-dependent apoptosis and its ability to promote apoptosis in transiently transfected cells, raises the possibility that it plays some role in facilitating the apoptotic activities of p53. Monoclonal antibodies reacting specifically with PAG608 may be applied in diverse cellular and molecular approaches for the study of nuclear processes and to elucidate the nature of the involvement and activity of zinc finger proteins.

General readings

Hellborg F., et al. (2001) Oncogene. 20:5466-5474Mendez Vidal C., et al. (2006) FEBS Lett. 580:4401-4408

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ZMAT3 antibody

WB Suggested Anti-ZMAT3 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ZMAT3 antibody

Western Blot analysis of ZMAT3 expression in transfected 293T cell line (H00064393-T01) by ZMAT3 MaxPab polyclonal antibody.

Lane 1: WIG1 transfected lysate(31.9 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

ZMAT3 antibody

Western Blot analysis of ZMAT3 expression in transfected 293T cell line (H00064393-T01) by ZMAT3 MaxPab polyclonal antibody.

Lane 1: WIG1 transfected lysate(31.9 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

ZMAT3 antibody

Immunofluorescence of purified MaxPab antibody to ZMAT3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

ZMAT3 antibody

Western Blot analysis of ZMAT3 expression in transfected 293T cell line (H00064393-T03) by ZMAT3 MaxPab polyclonal antibody.

Lane 1: ZMAT3 transfected lysate(32.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

ZMAT3 antibody

Western Blot analysis of ZMAT3 expression in transfected 293T cell line (H00064393-T03) by ZMAT3 MaxPab polyclonal antibody.

Lane 1: ZMAT3 transfected lysate(32.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ZMAT3 antibody

Human Muscle; WB Suggested Anti-ZMAT3 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Muscle; ZMAT3 antibody - N-terminal region (ARP50793_P050) in Human Muscle cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

ZMAT3 antibody

Western blot analysis of ZMAT3 expression in HEK293T (A); Raw264.7 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ZMAT3 antibody

Western blot analysis of ZMAT3 expression in HEK293T (A); Raw264.7 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ZMAT3 antibody

Western blot analysis of ZMAT3 expression in HEK293T (A); Raw264.7 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

ZMAT3 antibody

Immunohistochemistry: PAG608 Antibody [NBP1-32543] - analysis of paraffin-embedded OVCAR3 xenograft , using WIG1 antibody at 1:100 dilution. Rabbit IgG Hu ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ZMAT3

ZMAT3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ZMAT3 (1-289, His-tag)

ZMAT3 Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €730.00
  Acris Antibodies GmbH

ZMAT3 (1-289, His-tag)

ZMAT3 Human Purified > 85 % by SDS - PAGE E. coli
20 µg / €300.00
  Acris Antibodies GmbH

ZMAT3

ZMAT3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

ZMAT3

ZMAT3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

ZMAT3

ZMAT3 Human
2 µg / €300.00
  Abnova Taiwan Corp.

ZMAT3

ZMAT3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

ZMAT3

ZMAT3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

ZMAT3 (Human) IP-WB Antibody Pair

ZMAT3 (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

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