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ZNF36 antibody

Principal name

ZNF36 antibody

Alternative names for ZNF36 antibody

ZNF139, Zinc finger protein 36, zinc finger protein 139, ZKSCAN1, KOX18, Zinc finger protein with KRAB and SCAN domains 1, Zinc finger protein KOX18

SwissProt ID

P17029 (Human), Q4KLI1 (Rat), Q8BGS3 (Mouse), Q8BGS3-2

Gene ID

7586 (ZKSCAN1), 74570 (Zkscan1), 117586 (A1bg)

Ncbi ID

9606, NP_003430, NM_029869, NP_003430.1, NM_003439, NP_084145.1

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of ZNF36 antibody

The ZKSCAN1 gene encodes a transcriptiol regulator of the KRAB (Kruppel-associated box) subfamily of zinc finger proteins, which contain repeated Cys2-His2 (C2H2) zinc finger domains that are connected by conserved sequences, called H/C links (summarized by Tommerup and Vissing, 1995 [PubMed 7557990]). Transcriptiol regulatory proteins containing tandemly repeated zinc finger domains are thought to be involved in both normal and abnormal cellular proliferation and differentiation. See ZNF91 (MIM 603971) for general information on zinc finger proteins.[supplied by OMIM, Jul 2010]. Transcript Variant: This variant (3) differs in its 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (c) is shorter at the N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordites used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combition :: AK091471.1, DA499270.1 [ECO:0000332] Rseq introns :: single sample supports all introns ERS025081 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

General readings

Scherer,S.W., (2003) Science 300 (5620), 767-772

Western Blot analysis of ZKSCAN1 expression in transfected 293T cell line (H00007586-T01) by ZKSCAN1 MaxPab polyclonal antibody.Lane 1: ZKSCAN1 transfected lysate(61.93 KDa).Lane 2: Non-transfected lysate.

H00007586-B01

H00007586-B01P

Western Blot: ZKSCAN1 Antibody [NBP1-00102] - Detection of Human ZKSCAN1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZKSCAN1 antibody NBP1-00102 used for IP at 3 mcg/mg lysate. ZKSCAN1 was also immunoprecipitated by rabbit anti-ZKSCAN1 antibody NBP1-00103, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds.

NBP1-00102

Western Blot and Immunoprecipitation: ZKSCAN1 Antibody [NBP1-00103] - Detection of Human ZKSCAN1 on HeLa whole cell lysate using NBP1-00103. ZKSCAN1 was also immunoprecipitated by rabbit anti-ZKSCAN1 antibody NBP1-00102.

NBP1-00103

Human Jurkat; WB Suggested Anti-ZKSCAN1 Antibody Titration: 5.0ug/ml. Positive Control: Jurkat cell lysate; ZKSCAN1 antibody - N-terminal region (ARP47579_T100) in Human Jurkat cells using Western Blot

ARP47579_T100

Western Blot: ZKSCAN1 Lysate [NBL1-18036] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for ZKSCAN1

NBL1-18036

Human Brain; WB Suggested Anti-ZKSCAN1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; ZKSCAN1 antibody - middle region (ARP47580_P050) in Human Brain cells using Western Blot

ARP47580_P050

Mouse Heart; WB Suggested Anti-Zkscan1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Heart; Zkscan1 antibody - C-terminal region (ARP33524_P050) in Mouse Heart cells using Western Blot

ARP33524_P050

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZKSCAN1(GTX114888) antibody at 1:500 dilution.

GTX114888

Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE GTX114888 diluted at 1:1000

GTX114888

Immunohistochemistry-Paraffin: ZKSCAN1 Antibody [NBP1-81346] - Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.

NBP1-81346

Immunofluorescence: ZKSCAN1 Antibody [NBP1-81346] - 2 OS shows positivity in nucleus & mitochondria.

NBP1-81346

NBP1-81346

HeLa cells were subjected to SDS PAGE followed by western blot with 25214-1-AP( ZKSCAN1 Antibody) at dilution of 1:600

25214-1-AP

PC-3 cells were subjected to SDS PAGE followed by western blot with 25214-1-AP( ZKSCAN1 Antibody) at dilution of 1:600

25214-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 25214-1-AP( ZKSCAN1 Antibody) at dilution of 1:600

25214-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 25214-1-AP( ZKSCAN1 Antibody) at dilution of 1:600

25214-1-AP

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 25214-1-AP( ZKSCAN1 Antibody) at dilution of 1:600

25214-1-AP

TP760748

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA334001	ZNF36 antibody
	Rabbit	IgG		Purified	Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze	WB	50 µg / €325.00
							OriGene Technologies, Inc.
TA342429	ZNF36 antibody
	Rabbit	IgG		Purified	Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt	WB	0.1 mg / €325.00
							OriGene Technologies, Inc.
TA342430	ZNF36 antibody
	Rabbit	IgG		Purified	Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt	WB	50 µg / €325.00
							OriGene Technologies, Inc.
H00007586-B01	ZNF36 antibody
	Mouse			Serum	Hu	E(detection), WB	50 µl / €370.00
							Abnova Taiwan Corp.
H00007586-B01P	ZNF36 antibody
	Mouse			Purified	Hu	E(detection), WB	50 µg / €370.00
							Abnova Taiwan Corp.
H00007586-M03	ZNF36 antibody
	Mouse	IgG2a	2C7	Purified	Hu	E, WB	0.1 mg / €370.00
							Abnova Taiwan Corp.
ARP33524_P050	ZNF36 (C-term) antibody
	Rabbit			Aff - Purified	Hu, Ms	WB	50 µg / €350.00
							AVIVA Systems Biology
ARP47579_T100	ZNF36 antibody
	Rabbit			Aff - Purified	Bov, Can, Hu, Ms, Rt	WB	0.1 mg / €310.00
							AVIVA Systems Biology
ARP47580_P050	ZNF36 antibody
	Rabbit			Aff - Purified	Bov, Hu, Ms, Rt	WB	50 µg / €350.00
							AVIVA Systems Biology
NBP1-00102	ZNF36 antibody
	Rabbit	IgG		Aff - Purified	Hu	IP, WB	0.1 ml / €440.00
							Novus Biologicals Inc.
NBP1-00103	ZNF36 antibody
	Rabbit	IgG		Aff - Purified	Hu	IP, WB	0.1 ml / €440.00
							Novus Biologicals Inc.
NBP1-81346	ZNF36 antibody
	Rabbit	IgG		Aff - Purified	Hu	ICC/IF, P, WB	0.1 ml / €500.00
							Novus Biologicals Inc.
+2 additional images

Proteins & Growth Factors

Catalog No.	Species	Pres.	Purity	Source
TP760748	ZNF36 (full length, N-term HIS tag)
	Human		> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	50 µg / €199.00
					OriGene Technologies, Inc.
H00007586-P01-10	ZNF36
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.		10 µg / €380.00
					Abnova Taiwan Corp.
H00007586-P01-25	ZNF36
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.		25 µg / €510.00
					Abnova Taiwan Corp.
H00007586-P02	ZNF36
	Human	Purified			10 µg / €380.00
					Abnova Taiwan Corp.
H00007586-P02-10	ZNF36
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.		10 µg / €380.00
					Abnova Taiwan Corp.
H00007586-P02-25	ZNF36
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.		25 µg / €510.00
					Abnova Taiwan Corp.

Lysates

Catalog No.
H00007586-T01	ZKSCAN1 293T Cell Transient Overexpression Lysate(Denatured)
		0.1 ml / €270.00
		Abnova Taiwan Corp.

Lysates (Overexpressed)

Catalog No.
LY418696	ZKSCAN1 overexpression lysate
		0.1 mg / €280.00
		OriGene Technologies, Inc.

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