ZO-1 / Tight junction protein 1 antibody

Principal name

ZO-1 / Tight junction protein 1 antibody

Alternative names for ZO-1 / Tight junction protein 1 antibody

Zo1, Tjp1, Zonula occludens protein 1, Zona occludens protein 1

SwissProt ID

P39447 (Mouse), Q07157 (Human)

Gene ID

7082 (TJP1)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Chk (Chicken), Rt (Rat), Mky (Monkey), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse, Guinea Pig

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Frozen Sections (C), Flow Cytometry (F)

Background of ZO-1 / Tight junction protein 1 antibody

The polyclonal antibody recognizes human zona occludens 1 (ZO-1), an ~220 kDa tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. ZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties. ZO-1 immunoprecipitates with its family member ZO-2. ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling. Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene. The ZO-1 alpha-plus contains an 80 amino acids motif called alpha which is not present in ZO-1 alpha-minus. The alpha-containing isoform is found in most epithelial cell junctions. The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. This difference in distribution provides molecular distinction among tight junctions.

General readings

Zhong Z, Deane R, Ali Z, Parisi M, Shapovalov Y, O\1Banion MK, Stojanovic K, Sagare A, Boillee S, Cleveland DW, Zlokovic BV, ALS-causing SOD1 mutants generate vascular changes prior to motor neuron degeneration. Nat. Neurosci. 2008 Apr 11 (4): 420-2.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ZO-1 / Tight junction protein 1 antibody

Sample(30 ug whole cell lysate). A:H1299. 5% SDS PAGE. TA308455 diluted at 1:3000 Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ZO-1 / Tight junction protein 1 antibody

Sample (30 ug of whole cell lysate). @A: HCT116. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ZO-1 / Tight junction protein 1 antibody

(0.3ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ZO-1 / Tight junction protein 1 antibody

WB Suggested Anti-TJP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ZO-1 / Tight junction protein 1 antibody

Western blot analysis of ZO 1 expression in Human brain Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ZO-1 / Tight junction protein 1 (C-term) antibody

ZO-1 / Tight junction protein 1 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
20 µl / €50.00
  SICGEN

ZO-1 / Tight junction protein 1 (C-term) antibody

ZO-1 / Tight junction protein 1 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
0.6 mg / €320.00
  SICGEN

ZO-1 / Tight junction protein 1 antibody

ZO-1 / Tight junction protein 1 Rabbit Ig Purified Can, Hu, Rt F, ICC/IF, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

ZO-1 / Tight junction protein 1 (alpha plus) antibody

ZO-1 / Tight junction protein 1 Rabbit Ig Purified Can, Hu, Ms, Rt C, F, ICC/IF, P, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

ZO-1 / Tight junction protein 1 (alpha minus) antibody

ZO-1 / Tight junction protein 1 Guinea Pig Ig Purified Can, Hu, Ms, Rt C, ICC/IF, P, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

ZO-1 / Tight junction protein 1 antibody

ZO-1 / Tight junction protein 1 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

ZO-1 / Tight junction protein 1 antibody

Human Testis; Testis; TJP1 antibody - middle region (ARP36636_P050) in Human Testis cells using Immunohistochemistry Rabbit Aff - Purified Can, Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

ZO-1 / Tight junction protein 1 antibody

ZO-1 / Tight junction protein 1 Goat Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

ZO-1 / Tight junction protein 1 antibody

ZO-1 / Tight junction protein 1 Guinea Pig IgG Aff - Purified Can, Hu, Ms, Rt C, ICC/IF, P, WB
0.1 mg / €670.00
  Novus Biologicals Inc.

ZO-1 / Tight junction protein 1 antibody

ZO-1 / Tight junction protein 1 Guinea Pig IgG Aff - Purified Can, Hu, Ms, Rt C, ICC/IF, P, WB
50 µg / €400.00
  Novus Biologicals Inc.

ZO-1 / Tight junction protein 1 antibody

ZO-1 / Tight junction protein 1 Mouse IgM 7H6 Purified Can, Chk, Hu, Ms, Rt C, P, WB
25 µg / €380.00
  Novus Biologicals Inc.

ZO-1 / Tight junction protein 1 antibody

Immunohistochemistry-Paraffin: ZO-1 tight junction protein Antibody [NBP1-85046] - Immunohistochemical staining of human kidney shows distinct membranous and cytoplasmic positivity in cells of glomeruli. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

ZO-1 / Tight junction protein 1 antibody

Immunohistochemistry-Paraffin: ZO-1 tight junction protein Antibody [NBP1-85047] - Immunohistochemical staining of human kidney shows distinct membranous positivity in cells in glomeruli. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ZO-1 / Tight junction protein 1 (transcript variant 2)

ZO-1 / Tight junction protein 1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ZO-1 / Tight junction protein 1 (transcript variant 1)

ZO-1 / Tight junction protein 1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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