ZWILCH antibody

Principal name

ZWILCH antibody

Alternative names for ZWILCH antibody

hZwilch, Protein zwilch homolog

SwissProt ID

A6QM04 (Bovin), Q8R060 (Mouse), Q9H900 (Human)

Gene ID

55055 (ZWILCH)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of ZWILCH antibody

Zwilch is the human homolog of the Drosophila Zwilch protein. The Drosophila Zwilch forms a complex with both ROD Rough Deal) and ZWINT (Zeste-White 10, also designated ZW10) proteins. This complex is important for chromosome segregation because it recruits cytoplasmic Dynein to the kinetochore and plays a crucial role in the spindle checkpoint. The role of Zwilch in complex is thought to be evolutionarily conserved because the human homologs of Zwilch, ZWINT and ROD coimmunoprecipitate in a human cell line called HeLa. The human Zwilch, ZWINT and ROD complex localizes to the kinetochores at prometaphase. Mutations were discovered in Zwilch, ZWINT and ROD during a screen for mutations in alleles encoding putative chromosome instability genes in cases of human colorectal cancer. These mutations may contribute in part to the chromosomal instability phenotype of colorectal tumor cells.

General readings

Wang,C., et al. (2008) . Biol. Chem. 283 (17), 11565-11574
Meng,F., et al. (2003) Brain Res. 967 (1-2), 161-169

14 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ZWILCH antibody

Predicted band size: 67 kDa. Positive control: HT-29 and hela cell lysate. Recommended dilution: 1/1000-5000. (Gel: 8%SDS-PAGE Lane1: HT-29 cell lysate Lane2: HT-29 cell lysate Lysates: 40 ug per lane Primary antibody: 1/1750 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 seconds) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ZWILCH (C-term) antibody

Western blot analysis in mouse spleen tissue lysates (35ug/lane) using ZWILCH Antibody (C-term) (Cat.-No AP33477PU-N). This demonstrates the ZWILCH antibody detected the ZWILCH protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ZWILCH antibody

Western Blot analysis of ZWILCH expression in transfected 293T cell line (H00055055-T01) by ZWILCH MaxPab polyclonal antibody.

Lane 1: ZWILCH transfected lysate(52.47 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

ZWILCH antibody

ZWILCH Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ZWILCH antibody

Western Blot analysis of ZWILCH expression in transfected 293T cell line by ZWILCH monoclonal antibody (M01), clone 1C9.

Lane 1: ZWILCH transfected lysate(54.224 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 1C9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ZWILCH antibody

ZWILCH Mouse IgG1 1C10 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

ZWILCH antibody

Western Blot: ZWILCH Antibody [NBP1-49690]-Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.  Affinity purified rabbit anti-Zwilch antibody used for IP at 3 mcg/mg lysate.  Zwilch was also immunoprecipitated by another rabbit anti-Zwilch antibody, which recognizes an upstream epitope. Rabbit Aff - Purified Hu IP
0.1 ml / €440.00
  Novus Biologicals Inc.

ZWILCH antibody

Immunohistochemistry-Paraffin: ZWILCH Antibody [NBP1-84651] - Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ZWILCH (1-591, His-tag)

ZWILCH Human Purified > 85 % by SDS - PAGE E. coli
0.25 mg / €730.00
  Acris Antibodies GmbH

ZWILCH (1-591, His-tag)

ZWILCH Human Purified > 85 % by SDS - PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

ZWILCH

ZWILCH Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ZWILCH

ZWILCH Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ZWILCH 293T Cell Transient Overexpression Lysate(Denatured)

ZWILCH 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

Lysates (Overexpressed)

Catalog No.    

ZWILCH overexpression lysate

ZWILCH overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

14 Item(s)

per page
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